pro-siRNA technology is a method of producing
highly potent and cost-effective small interfering RNAs in bacteria
This technology was originally developed by the Lieberman Laboratory from Boston Children’s Hospital and is patent protected.
Dr. Linfeng Huang's lab at City University of Hong Kong is currently further developing this technology.
US patent 9,840,703
1. Huang et al, Efficient and specific gene knockdown by small interfering RNAs produced in bacteria, Nature Biotechnology, 31, 350-356, Apr 2013 PubMed
2. Huang & Lieberman, Production of highly potent recombinant siRNAs in Escherichia coli, Nature Protocols, Dec 2013 PubMed
4. Blau & McManus, Renewable RNAi, Nature Biotechnology 31, 319–320, Apr 2013 PubMed
5. Bacterial synthesis of small interfering RNA, Science-Business eXchange 6, doi:10.1038/scibx.2013.274