pro-siRNA technology is a method of producing
highly potent and cost-effective small interfering RNAs in bacteria
This technology was originally developed at Boston Children’s Hospital.
US patent 9,840,703
Xiaomo Biotech Limited is commercializing this technology at Hong Kong Science Park.
1. Huang et al., Efficient and specific gene knockdown by small interfering RNAs produced in bacteria, Nature Biotechnology, 31, 350-356, Apr 2013 PubMed
2. Huang & Lieberman, Production of highly potent recombinant siRNAs in Escherichia coli, Nature Protocols, Dec 2013 PubMed
4. Blau & McManus, Renewable RNAi, Nature Biotechnology 31, 319–320, Apr 2013 PubMed
5. Bacterial synthesis of small interfering RNA, Science-Business eXchange 6, doi:10.1038/scibx.2013.274
6. Huang et al., Tombusvirus p19 captures RNase III-cleaved double-stranded RNAs formed by overlapping sense and antisense transcripts in E. coli, mBio, 11(3):e00485-20, Jun 2020 Link to the article