pro-siRNA technology is a method of producing highly potent and cost-effective small interfering RNAs in bacteria  

This technology was originally developed by the Lieberman Laboratory from Boston Children’s Hospital and is patent protected.

Dr. Linfeng Huang's lab at City University of Hong Kong is currently further developing this technology.


Prof. Judy Lieberman, Dr. Linfeng Huang


US patent 9,840,703

Licensing information:

Technology and Innovation Development Office (TIDO), Boston Children's Hospital


Key references:

1. Huang et al, Efficient and specific gene knockdown by small interfering RNAs produced in bacteria, Nature Biotechnology, 31, 350-356, Apr 2013 PubMed

2. Huang & Lieberman, Production of highly potent recombinant siRNAs in Escherichia coli, Nature Protocols, Dec 2013 PubMed

3. Guneet et al, Milligram scale production of potent recombinant small interfering RNAs in Escherichia coli, Biotechnology and Bioengineering, Jun 2018 Pubmed Read only version

4. Blau & McManus, Renewable RNAi, Nature Biotechnology 31, 319–320, Apr 2013 PubMed

5. Bacterial synthesis of small interfering RNA, Science-Business eXchange 6, doi:10.1038/scibx.2013.274