pro-siRNA technology is a method of producing highly potent and cost-effective small interfering RNAs in bacteria  

This technology was originally developed at Boston Children’s Hospital.

US patent 9,840,703


Prof. Judy Lieberman, Prof. Linfeng Huang


Xiaomo Biotech Limited is commercializing this technology at Hong Kong Science Park.


Key references:

1. Huang et al., Efficient and specific gene knockdown by small interfering RNAs produced in bacteria, Nature Biotechnology, 31, 350-356, Apr 2013 PubMed

2. Huang & Lieberman, Production of highly potent recombinant siRNAs in Escherichia coli, Nature Protocols, Dec 2013 PubMed

3. Guneet et al., Milligram scale production of potent recombinant small interfering RNAs in Escherichia coli, Biotechnology and Bioengineering, Jun 2018 Pubmed Read only version

4. Blau & McManus, Renewable RNAi, Nature Biotechnology 31, 319–320, Apr 2013 PubMed

5. Bacterial synthesis of small interfering RNA, Science-Business eXchange 6, doi:10.1038/scibx.2013.274

6. Huang et al., Tombusvirus p19 captures RNase III-cleaved double-stranded RNAs formed by overlapping sense and antisense transcripts in E. coli, mBio, 11(3):e00485-20, Jun 2020 Link to the article